Author:
Belova Vera,Pavlova Anna,Afasizhev Robert,Moskalenko Viktoriya,Korzhanova Margarita,Krivoy Andrey,Cheranev Valery,Nikashin Boris,Bulusheva Irina,Rebrikov Denis,Korostin Dmitriy
Abstract
AbstractHuman exome sequencing is a classical method used in most medical genetic applications. The leaders in the field are the manufacturers of enrichment kits based on hybridization of cRNA or cDNA biotinylated probes specific for a genomic region of interest. Recently, the platforms manufactured by the Chinese company MGI Tech have become widespread in Europe and Asia. The reliability and quality of the obtained data are already beyond any doubt. However, only a few kits compatible with these sequencers can be used for such specific tasks as exome sequencing. We developed our own solution for library pre-capture pooling and exome enrichment with Agilent probes. In this work, using a set of the standard benchmark samples from the Platinum Genome collection, we demonstrate that the qualitative and quantitative parameters of our protocol which we called “RSMU_exome” exceed those of the MGI Tech kit. Our protocol allows for identifying more SNV and indels, generates fewer PCR duplicates, enables pooling of more samples in a single enrichment procedure, and requires less raw data to obtain results comparable with the MGI Tech's protocol. The cost of our protocol is also lower than that of MGI Tech's solution.
Funder
Ministry of Science and Higher Education of the Russian Federation
Publisher
Springer Science and Business Media LLC
Cited by
8 articles.
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