Author:
García-Vidal José Antonio,Salinas Jesús,Ortega Nieves,Escolar-Reina Pilar,Camacho-Alonso Fabio,Medina-Mirapeix Francesc
Abstract
AbstractPercutaneous needle electrolysis using tri-beveled needles with a specific protocol (5 mA applied for 25 s) has demonstrated to provoke a clinical reduction of recurrent bacterial infections in mammary fistulas. However, the bactericidal effect of needle electrolysis in this pathology remains theoretical. This in vitro study evaluated the bactericidal effect of this protocol and whether it changed when introducing small variations. Staphylococcus aureus were generated in saline solution (9 Log10 CFU/mL) and treated in three different experiments including the main protocol and introducing variations in needle gauge, intensity, and total dosage, respectively. After 24 h, the viable cell count showed that the protocol had an average reduction of 5 log10 CFU/ml compared to the control group. While variations in needle gauge did not modify this effect, variations in current intensity or dosage did. This study demonstrated that the bacterial effect was greater by increasing either current intensity or total dosage, and it decreased with substantial reductions of these parameters.
Publisher
Springer Science and Business Media LLC
Reference22 articles.
1. Cheng, X. et al. Bactericidal effect of strong acid electrolyzed water against flow enterococcus faecalis biofilms. J. Endodontics 42, 1120–1125. https://doi.org/10.1016/j.joen.2016.04.009 (2016).
2. Ogawa, T. Applications and Theory of Electrolyzed Acidic Water (SLI Pub, 1995).
3. Shimizu, Y. & Furusawa, T. Killing action of virus, bacteria and fungus by oxidative potential water induced by electrolysis. J. Dent. Med. 36, 1055–1060 (1992).
4. Shimizu, Y. et al. Disinfectant action of electrolyzed oxidizing water to dental instruments and finger. J. Dent. Med. 40, 905–911 (1994).
5. Kubota, A. et al. Efficacy and safety of strong acid electrolyzed water for peritoneal lavage to prevent surgical site infection in patients with perforated appendicitis. Surg. Today 45, 876–879. https://doi.org/10.1007/s00595-014-1050-x (2015).