Author:
Yang Yunzhi Peter,Labus Kevin M.,Gadomski Benjamin C.,Bruyas Arnaud,Easley Jeremiah,Nelson Brad,Palmer Ross H.,McGilvray Kirk,Regan Daniel,Puttlitz Christian M.,Stahl Alexander,Lui Elaine,Li Jiannan,Moeinzadeh Seyedsina,Kim Sungwoo,Maloney William,Gardner Michael J.
Abstract
AbstractAutologous bone grafts are considered the gold standard grafting material for the treatment of nonunion, but in very large bone defects, traditional autograft alone is insufficient to induce repair. Recombinant human bone morphogenetic protein 2 (rhBMP-2) can stimulate bone regeneration and enhance the healing efficacy of bone grafts. The delivery of rhBMP-2 may even enable engineered synthetic scaffolds to be used in place of autologous bone grafts for the treatment of critical size defects, eliminating risks associated with autologous tissue harvest. We here demonstrate that an osteoinductive scaffold, fabricated by combining a 3D printed rigid polymer/ceramic composite scaffold with an rhBMP-2-eluting collagen sponge can treat extremely large-scale segmental defects in a pilot feasibility study using a new sheep metatarsus fracture model stabilized with an intramedullary nail. Bone regeneration after 24 weeks was evaluated by micro-computed tomography, mechanical testing, and histological characterization. Load-bearing cortical bridging was achieved in all animals, with increased bone volume observed in sheep that received osteoinductive scaffolds compared to sheep that received an rhBMP-2-eluting collagen sponge alone.
Funder
National Institute of Arthritis and Musculoskeletal and Skin Diseases
U.S. Department of Defense
Publisher
Springer Science and Business Media LLC
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