Author:
Ecclestone Benjamin R.,Bell Kevan,Abbasi Saad,Dinakaran Deepak,van Landeghem Frank K. H.,Mackey John R.,Fieguth Paul,Haji Reza Parsin
Abstract
AbstractMalignant brain tumors are among the deadliest neoplasms with the lowest survival rates of any cancer type. In considering surgical tumor resection, suboptimal extent of resection is linked to poor clinical outcomes and lower overall survival rates. Currently available tools for intraoperative histopathological assessment require an average of 20 min processing and are of limited diagnostic quality for guiding surgeries. Consequently, there is an unaddressed need for a rapid imaging technique to guide maximal resection of brain tumors. Working towards this goal, presented here is an all optical non-contact label-free reflection mode photoacoustic remote sensing (PARS) microscope. By using a tunable excitation laser, PARS takes advantage of the endogenous optical absorption peaks of DNA and cytoplasm to achieve virtual contrast analogous to standard hematoxylin and eosin (H&E) staining. In conjunction, a fast 266 nm excitation is used to generate large grossing scans and rapidly assess small fields in real-time with hematoxylin-like contrast. Images obtained using this technique show comparable quality and contrast to the current standard for histopathological assessment of brain tissues. Using the proposed method, rapid, high-throughput, histological-like imaging was achieved in unstained brain tissues, indicating PARS’ utility for intraoperative guidance to improve extent of surgical resection.
Funder
Natural Sciences and Engineering Research Council of Canada
Canadian Foundation for Innovation
University of Waterloo Startup Funds
University of Waterloo, Centre for Bioengineering and Biotechnology
illumiSonics Inc
New Frontiers in Research Fund – Exploration
Publisher
Springer Science and Business Media LLC
Cited by
25 articles.
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