Light dependent synthesis of a nucleotide second messenger controls the motility of a spirochete bacterium

Author:

Xu Jun,Koizumi Nobuo,Morimoto Yusuke V.,Ozuru Ryo,Masuzawa Toshiyuki,Nakamura Shuichi

Abstract

AbstractNucleotide second messengers are universally crucial factors for the signal transduction of various organisms. In prokaryotes, cyclic nucleotide messengers are involved in the bacterial life cycle and in functions such as virulence and biofilm formation, mainly via gene regulation. Here, we show that the swimming motility of the soil bacterium Leptospira kobayashii is rapidly modulated by light stimulation. Analysis of a loss-of-photoresponsivity mutant obtained by transposon random mutagenesis identified the novel sensory gene, and its expression in Escherichia coli through codon optimization elucidated the light-dependent synthesis of cyclic adenosine monophosphate (cAMP). GFP labeling showed the localization of the photoresponsive enzyme at the cell poles where flagellar motors reside. These findings suggest a new role for cAMP in rapidly controlling the flagella-dependent motility of Leptospira and highlight the global distribution of the newly discovered photoactivated cyclase among diverse microbial species.

Funder

Japan Society for the Promotion of Science

JST PRESTO

Publisher

Springer Science and Business Media LLC

Subject

Multidisciplinary

Cited by 4 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Genomic insights into the c-di-GMP signaling and biofilm development in the saprophytic spirochete Leptospira biflexa;Archives of Microbiology;2023-04-08

2. Diving into the complexity of the spirochetal endoflagellum;Trends in Microbiology;2023-03

3. John Buridan;American Catholic Philosophical Quarterly;2023

4. Frontiers of microbial movement research;Biophysics and Physicobiology;2023

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