Author:
Könemund Lea,Neumann Laurie,Hirschberg Felix,Biedendieck Rebekka,Jahn Dieter,Johannes Hans-Hermann,Kowalsky Wolfgang
Abstract
AbstractTraditional sensing technologies have drawbacks as they are time-consuming, cost-intensive, and do not attain the required accuracy and reproducibility. Therefore, new methods of measurements are necessary to improve the detection of bacteria. Well-established electrical measurement methods can connect high sensitive sensing systems with biological requirements. One approach is to functionalize an extended-gate field-effect transistor’s (EGFET) sensing area with modified porphyrins containing two different linkers. One linker connects the electrode surface with the porphyrin. The other linker bonds bacteria on the functional layer through a specific peptide chain. The negative charge on the surface of the cells regulates the surface potential which has an impact on the electrical behavior of the EGFET. The attendance of attached bacteria on the functionalized sensing area could successfully be detected.
Funder
Volkswagen Foundation for funding Project Laboratory Across Borders
Lower Saxony Ministry for Science and Culture (MWK) for funding the Quantum- and Na-nometrology
Technische Universität Braunschweig
Publisher
Springer Science and Business Media LLC
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