Author:
Tanikawa Satoshi,Ebisu Yuki,Sedlačík Tomáš,Semba Shingo,Nonoyama Takayuki,Kurokawa Takayuki,Hirota Akira,Takahashi Taiga,Yamaguchi Kazushi,Imajo Masamichi,Kato Hinako,Nishimura Takuya,Tanei Zen-ichi,Tsuda Masumi,Nemoto Tomomi,Gong Jian Ping,Tanaka Shinya
Abstract
AbstractNeural regeneration is extremely difficult to achieve. In traumatic brain injuries, the loss of brain parenchyma volume hinders neural regeneration. In this study, neuronal tissue engineering was performed by using electrically charged hydrogels composed of cationic and anionic monomers in a 1:1 ratio (C1A1 hydrogel), which served as an effective scaffold for the attachment of neural stem cells (NSCs). In the 3D environment of porous C1A1 hydrogels engineered by the cryogelation technique, NSCs differentiated into neuroglial cells. The C1A1 porous hydrogel was implanted into brain defects in a mouse traumatic damage model. The VEGF-immersed C1A1 porous hydrogel promoted host-derived vascular network formation together with the infiltration of macrophages/microglia and astrocytes into the gel. Furthermore, the stepwise transplantation of GFP-labeled NSCs supported differentiation towards glial and neuronal cells. Therefore, this two-step method for neural regeneration may become a new approach for therapeutic brain tissue reconstruction after brain damage in the future.
Funder
Ministry of Education, Culture, Sports, Science and Technology
Japan Agency for Medical Research and Development
Publisher
Springer Science and Business Media LLC
Cited by
4 articles.
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