Establishment of a modified CRISPR/Cas9 system with increased mutagenesis frequency using the translational enhancer dMac3 and multiple guide RNAs in potato-Reference-Cited by-同舟云学术

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Establishment of a modified CRISPR/Cas9 system with increased mutagenesis frequency using the translational enhancer dMac3 and multiple guide RNAs in potato

Published:2018-09-13 Issue:1 Volume:8 Page:
ISSN:2045-2322
Container-title:Scientific Reports
language:en
Short-container-title:Sci Rep

Author:

Kusano Hiroaki,Ohnuma Mariko,Mutsuro-Aoki Hiromi,Asahi Takahiro,Ichinosawa Dai,Onodera Hitomi,Asano Kenji,Noda Takahiro,Horie Takaaki,Fukumoto Kou,Kihira Miho,Teramura Hiroshi,Yazaki Kazufumi,Umemoto Naoyuki,Muranaka Toshiya,Shimada Hiroaki

Funder

Cross-ministerial Strategic Innovation Promotion Program

Publisher

Springer Science and Business Media LLC

Subject

Multidisciplinary

Link

http://www.nature.com/articles/s41598-018-32049-2.pdf

Reference26 articles.

1. Jinek, M. et al. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337, 816–821 (2012).

2. Malzahn, A., Lowder, L. & Qi, Y. Plant genome editing with TALEN and CRISPR. Cell Biosci. 7, 21 (2017).

3. Schiml, S., Fauser, F. & Puchta, H. CRISPR/Cas-mediated in planta gene targeting. Methods Mol. Biol. 1610, 3–11 (2017).

4. Mikami, M., Toki, S. & Endo, M. Comparison of CRISPR/Cas9 expression constructs for efficient targeted mutagenesis in rice. Plant Mol. Biol. 88, 561–572 (2015).

5. Roy, B. & von Arnim, A. G. Translational regulation of cytoplasmic mRNAs. The Arabidopsis Book 11, e0165 (2013).

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1. Genome Editing in Potato Using CRISPR/Cas Technology: Applications and Challenges;Genetically Modified Organisms [Working Title];2024-08-27

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