Author:
Ratuski A. S.,Améndola L.,Makowska I. J.,Weary D. M.
Abstract
AbstractLaboratory mice are typically housed in “shoebox” cages with limited opportunities to engage in natural behaviour. Temporary access to environments with increased space and complexity (playpens) may improve mouse welfare. Previous work by our group has shown that mice are motivated to access and use these environments, but it is unknown how other aspects of welfare are impacted. Female C57BL/6J, BALB/cJ, and DBA/2J mice (n = 21; 7 mice per strain) were housed in mixed-strain trios and given temporary access to a large playpen with their cage mates three times per week. Control mice (n = 21; 7 mice per strain) remained in their home cages. Home cage behaviour (development of stereotypic behaviour over time, aggression following cage-changing) and anxiety tests were used to assess how playpen access impacted welfare. Contrary to our predictions, we found increased time spent performing stereotypies in playpen mice; this difference may be related to negative emotional states, increased motivation to escape the home cage, or active coping strategies. Playpen access resulted in strain-dependent improvements in aggression and some measures of anxiety. Aggression was lower for C57BL/6J mice in the playpen treatment following cage changing than it was for C57BL/6J control mice, while playpen mice, and particularly the C57BL/6J strain, spent more time in the center of the open field test and produced fewer fecal boli during anxiety testing, supporting other research showing that strain differences play an important role in behaviour and stress resiliency.
Funder
Natural Sciences and Engineering Research Council of Canada
Animal Welfare Institute
Publisher
Springer Science and Business Media LLC
Reference52 articles.
1. Nip, E. et al. Why are enriched mice nice? Investigating how environmental enrichment reduces agonism in female C57BL/6, DBA/2, and BALB/c mice. Appl. Anim. Behav. Sci. 217, 73–82. https://doi.org/10.1016/j.applanim.2019.05.002 (2019).
2. Clipperton-Allen, A. E. et al. Long-term provision of environmental resources alters behavior but not physiology or neuroanatomy of male and female BALB/c and C57Bl/6 mice. J. Am. Assoc. Lab. Anim. Sci. 54, 718–730 (2015).
3. Fureix, C. et al. Stereotypic behaviour in standard non-enriched cages is an alternative to depression-like responses in C57BL/6 mice. Behav. Brain Res. 305, 186–190. https://doi.org/10.1016/j.bbr.2016.02.005 (2016).
4. Sherwin, C. M. & Olsson, I. A. S. Housing conditions affect self-administration of anxiolytic by laboratory mice. Anim. Welf. 13, 33–38 (2004).
5. Friske, J. E. & Gammie, S. C. Environmental enrichment alters plus maze, but not maternal defense performance in mice. Physiol. Behav. 85, 187–194. https://doi.org/10.1016/j.physbeh.2005.03.022 (2005).
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