Author:
Boshra Hani,Lorenzo Gema,Charro Diego,Moreno Sandra,Guerra Gabriel Soares,Sanchez Isbene,Garrido Joseba M.,Geijo Marivi,Brun Alejandro,Abrescia Nicola G. A.
Abstract
AbstractSchmallenberg virus (SBV), an arthropod-transmitted pathogenic bunyavirus, continues to be a threat to the European livestock industry, causing morbidity and mortality among young ruminant livestock. Here, we describe a novel SBV subunit vaccine, based on bacterially expressed SBV nucleoprotein (SBV-N) administered with a veterinary-grade Saponin adjuvant. When assayed in an IFNAR-/-mouse model, SBV-N with Saponin induced strong non-neutralizing broadly virus-reactive antibodies, decreased clinical signs, as well as significantly reduced viremia. Vaccination assays also suggest that this level of immune protection is cell mediated, as evidenced by the lack of neutralizing antibodies,as well as interferon-γ secretion observed in vitro. Therefore, based on these results, bacterially expressed SBV-N, co-administered with veterinary-grade Saponin adjuvant may serve as a promising economical alternative to current SBV vaccines, and warrant further evaluation in large ruminant animal models. Moreover, we propose that this strategy may be applicable to other bunyaviruses.
Funder
Marie Sklodowska Curie Actions programme
Spanish Ministerio de Ciencia, Innovación y Universidades
Basque Departamento de Desarrollo Económico e Infraestructura
La Caixa” Foundation INPhINIT ‘La Caixa’ fellowship
Publisher
Springer Science and Business Media LLC
Cited by
5 articles.
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