Author:
Shimazaki Kenichiro,Yajima Takehiro,Ichikawa Hiroyuki,Sato Tadasu
Abstract
AbstractGalanin (GAL) is a nociceptive transmitter or modulator in the trigeminal sensory system. In this study, GAL expression was investigated in the rat dura mater to demonstrate its possible function in headache using immunohistochemical techniques. The cerebral falx and cerebellar dura mater received abundant blood and nerve supply, and were significantly thicker compared to other portions in the cerebral dura mater. GAL-immunoreactivity was expressed by cell and nerve fiber profiles. Presumed macrophages and dendritic cells contained GAL-immunoreactivity, and co-expressed with CD11b-immunoreactivity. Many isolated and perivascular nerve fibers also showed GAL-immunoreactivity. In addition, GAL-immunoreactive nerve fibers were present in the vicinity of macrophages and dendritic cells with either GAL- or ED1-immunoreactivity. GAL-immunoreactive cells and nerve fibers were common in the cerebral falx and cerebellar dura mater and infrequent in other portions. And, GAL-immunoreactive nerve fibers usually co-expressed calcitonin gene-related peptide (CGRP)-immunoreactivity. In the trigeminal ganglion, a substantial proportion of sensory neurons innervating the dura mater contained GAL-immunoreactivity (mean ± SD, 3.4 ± 2.2%), and co-expressed CGRP-immunoreactivity (2.7 ± 2.1%). The present study may suggest that GAL is associated with nociceptive transduction or modulation in the dura mater. GAL also possibly plays a role in the immune mechanism of the dura mater.
Publisher
Springer Science and Business Media LLC
Reference54 articles.
1. Standring, S., Berkovitz, B. K. B., Collins, P. & Wigley, C. (eds.) Gray’s Anatomy: The Anatomical Basis of Clinical Practice, chapt 15, 39 ed. 275–285 (Elsevier, 2008).
2. Kiliç, T. & Akakin, A. Anatomy of cerebral veins and sinuses. Front. Neurol. Neurosci. 23, 4–15 (2008).
3. Fricke, B., Andres, K. H. & Von Düring, M. Nerve fibers innervating the cranial and spinal meninges: Morphology of nerve fiber terminals and their structural integration. Microsc. Res. Tech. 53, 96–105 (2001).
4. McMenamin, P. G. Distribution and phenotype of dendritic cells and resident tissue macrophages in the dura mater, leptomeninges, and choroid plexus of the rat brain as demonstrated in wholemount preparations. J. Comp. Neurol. 405, 553–562 (1999).
5. Schain, A. J. et al. Activation of pial and dural macrophages and dendritic cells by cortical spreading depression. Ann. Neurol. 83, 508–521 (2018).
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