The elucidation of plasma lipidome profiles during severe influenza in a mouse model

Author:

Ohno Marumi,Gowda Siddabasave Gowda B.,Sekiya Toshiki,Nomura Naoki,Shingai Masashi,Hui Shu-Ping,Kida Hiroshi

Abstract

AbstractAlthough influenza virus infection has been shown to affect lipid metabolism, details remain unknown. Therefore, we elucidated the kinetic lipid profiles of mice infected with different doses of influenza virus A/Puerto Rico/8/34 (H1N1) (PR8) by measuring multiple lipid molecular species using untargeted lipidomic analysis. C57BL/6 male mice were intranasally infected with PR8 virus at 50 or 500 plaque-forming units to cause sublethal or lethal influenza, respectively. Plasma and tissue samples were collected at 1, 3, and 6 days post-infection (dpi), and comprehensive lipidomic analysis was performed using high-performance liquid chromatography–linear trap quadrupole–Orbitrap mass spectrometry, as well as gene expression analyses. The most prominent feature of the lipid profile in lethally infected mice was the elevated plasma concentrations of phosphatidylethanolamines (PEs) containing polyunsaturated fatty acid (PUFA) at 3 dpi. Furthermore, the facilitation of PUFA-containing phospholipid production in the lungs, but not in the liver, was suggested by gene expression and lipidomic analysis of tissue samples. Given the increased plasma or serum levels of PUFA-containing PEs in patients with other viral infections, especially in severe cases, the elevation of these phospholipids in circulation could be a biomarker of infection and the severity of infectious diseases.

Funder

Japan Initiative for World-leading Vaccine Research and Development Centers

Japan Agency for Medical Research and Development

Hokkaido Bureau of Economy, Trade, and Industry

Japan Society for the Promotion of Science

Publisher

Springer Science and Business Media LLC

Subject

Multidisciplinary

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