Author:
Kazemi Tina,Huang Shuyan,Avci Naze G.,Waits Charlotte Mae K.,Akay Yasemin M.,Akay Metin
Abstract
AbstractNicotine and alcohol are two of the most commonly used and abused recreational drugs, are often used simultaneously, and have been linked to significant health hazards. Furthermore, patients diagnosed with dependence on one drug are highly likely to be dependent on the other. Several studies have shown the effects of each drug independently on gene expression within many brain regions, including the ventral tegmental area (VTA). Dopaminergic (DA) neurons of the dopamine reward pathway originate from the VTA, which is believed to be central to the mechanism of addiction and drug reinforcement. Using a well-established rat model for both nicotine and alcohol perinatal exposure, we investigated miRNA and mRNA expression of dopaminergic (DA) neurons of the VTA in rat pups following perinatal alcohol and joint nicotine–alcohol exposure. Microarray analysis was then used to profile the differential expression of both miRNAs and mRNAs from DA neurons of each treatment group to further explore the altered genes and related biological pathways modulated. Predicted and validated miRNA-gene target pairs were analyzed to further understand the roles of miRNAs within these networks following each treatment, along with their post transcription regulation points affecting gene expression throughout development. This study suggested that glutamatergic synapse and axon guidance pathways were specifically enriched and many miRNAs and genes were significantly altered following alcohol or nicotine–alcohol perinatal exposure when compared to saline control. These results provide more detailed insight into the cell proliferation, neuronal migration, neuronal axon guidance during the infancy in rats in response to perinatal alcohol/ or nicotine–alcohol exposure.
Publisher
Springer Science and Business Media LLC
Reference148 articles.
1. Bobo, J. K. & Husten, C. Sociocultural influences on smoking and drinking. Alcohol Res. Health. 24, 225–232 (2000).
2. Drobes, D. J. Concurrent alcohol and tobacco dependence mechanisms and treatment. Alcohol Res. Health. 26, 2 (2002).
3. De Leon, J. et al. Association between smoking and alcohol use in the general population: Stable and unstable odds ratios across two years in two different countries. Alcohol Alcohol. 42, 252–257 (2007).
4. Batel, P., Pessione, F., Maitre, C. & Rueff, B. Relationship between alcohol and tobacco dependencies among alcoholics who smoke. Addiction 90, 977–980 (1995).
5. Burton, S. M., Tiffany, S. T., Burton, S. A. & Tiffany, S. T. The effect of alcohol consumption on craving to smoke. Addiction 92, 15–26 (1997).
Cited by
17 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献