Author:
Pan Cuili,Wang Shuzhe,Yang Chaoyun,Hu Chunli,Sheng Hui,Xue Xiaoshu,Hu Honghong,Lei Zhaoxiong,Yang Mengli,Ma Yun
Abstract
AbstractThe Wnt family features conserved glycoproteins that play roles in tissue regeneration, animal development and cell proliferation and differentiation. For its functional diversity and importance, this family has been studied in several species, but not in the Bovinae. Herein we identified 19 Wnt genes in cattle, and seven other species of Bovinae, and described their corresponding protein properties. Phylogenetic analysis clustered the 149 Wnt proteins in Bovinae, and 38 Wnt proteins from the human and mouse into 12 major clades. Wnt genes from the same subfamilies shared similar protein motif compositions and exon–intron patterns. Chromosomal distribution and collinearity analysis revealed that they were conservative in cattle and five species of Bovinae. RNA-seq data analysis indicated that Wnt genes exhibited tissue-specific expression in cattle. qPCR analysis revealed a unique expression pattern of each gene during bovine adipocytes differentiation. Finally, the comprehensive analysis indicated that Wnt2B may regulate adipose differentiation by activating FZD5, which is worthy of further study. Our study presents the first genome-wide study of the Wnt gene family in Bovinae, and lays the foundation for further functional characterization of this family in bovine adipocytes differentiation.
Funder
National Natural Science Foundation of China
Leading Talents Fund in Science and Technology Innovation in Henan Province
Key Research and Talent Introduction Project of Ningxia Hui Autonomous Region
Science and Technology Innovation Team Projects of Ningxia Hui Autonomous Region
Publisher
Springer Science and Business Media LLC
Reference65 articles.
1. Lorzadeh, S., Kohan, L., Ghavami, S. & Azarpira, N. Autophagy and the Wnt signaling pathway: A focus on Wnt/beta-catenin signaling. Biochim. Biophys. Acta Mol. Cell Res. 1868, 118926 (2021).
2. Yao, L. et al. The effect of regulating the Wnt signaling pathway on the proliferation and differentiation of spermatogonial stem cells. Ann. Transl. Med. 8, 1003 (2020).
3. Taciak, B., Pruszynska, I., Kiraga, L., Bialasek, M. & Krol, M., Wnt signaling pathway in development and cancer. J. Physiol. Pharmacol. 69 (2018).
4. De, A. Wnt/Ca2+ signaling pathway: a brief overview. Acta Biochim. Biophys. Sin. (Shanghai) 43, 745 (2011).
5. Yamanaka, H. et al. JNK functions in the non-canonical Wnt pathway to regulate convergent extension movements in vertebrates. Embo. Rep. 3, 69 (2002).