Author:
Meng Xianhong,Fu Qiang,Luan Sheng,Luo Kun,Sui Juan,Kong Jie
Abstract
AbstractFenneropenaeus chinensisis one of the most important aquaculture species in China. Research on its genomic and genetic structure not only helps us comprehend the genetic basis of complex economic traits, but also offers theoretical guidance in selective breeding. In the present study, a genome survey sequencing was performed to generate a rough reference genome utilized for groping preliminary genome characteristics and facilitate linkage and quantitative trait locus (QTL) mapping. Linkage mapping was conducted using a reduced-representation sequencing method 2b-RAD. In total, 36,762 SNPs were genotyped from 273 progenies in a mapping family, and a high-resolution linkage map was constructed. The consensus map contained 12,884 markers and spanned 5257.81 cM with an average marker interval of 0.41 cM, which was the first high-resolution genetic map inF. chinensisto our knowledge. QTL mapping and association analysis were carried out in 29 characters including body size, sex and disease resistance. 87 significant QTLs were detected in several traits and they were also evaluated by association analysis. Results of this study provide us valuable suggestions in genetic improvement and breeding of new varieties and also lay a basic foundation for further application of cloning of economic genes in selective breeding program and marker-assisted selection.
Funder
National Natural Science Foundation of China
National Key Research and Development Program of China
China Agriculture Research System
Publisher
Springer Science and Business Media LLC
Reference56 articles.
1. Benzie, J. A. H., Kenway, M. & Trott, L. Estimates for the heritability of size in juvenile Penaeus monodon, prawns from half-sib matings. Aquaculture 152(1), 49–53 (1997).
2. Preston, N. P., Crocos, P. J., Keys, S. J., Coman, G. J. & Koenig, R. Comparative growth of selected and non-selected Kuruma shrimp Penaeus (Marsupenaeus) japonicus in commercial farm ponds; implications for broodstock production. Aquaculture 231(1–4), 73–82 (2004).
3. Arcos, F. G., Racotta, I. S. & Ibarra, A. M. Genetic parameter estimates for reproductive traits and egg composition in Pacific white shrimp Penaeus (Litopenaeus) vannamei. Aquaculture 236(1–4), 151–165 (2004).
4. Sui, J. et al. Genetic parameters and response to selection of harvest body weight of the Chinese shrimp Fenneropenaeus chinensis after five generations of multi-trait selection. Aquaculture 50(1), 1–15 (2015).
5. Wang, C. M. et al. A high-resolution linkage map for comparative genome analysis and QTL fine mapping in Asian seabass, Lates calcarifer. BMC Genom. 12(1), 174 (2011).
Cited by
7 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献