Author:
Blakebrough-Hall C.,Dona A.,D’occhio M. J.,McMeniman J.,González L. A
Abstract
AbstractCurrent diagnosis methods for Bovine Respiratory Disease (BRD) in feedlots have a low diagnostic accuracy. The current study aimed to search for blood biomarkers of BRD using 1H NMR metabolomics and determine their accuracy in diagnosing BRD. Animals with visual signs of BRD (n = 149) and visually healthy (non-BRD; n = 148) were sampled for blood metabolomics analysis. Lung lesions indicative of BRD were scored at slaughter. Non-targeted 1H NMR metabolomics was used to develop predictive algorithms for disease classification using classification and regression trees. In the absence of a gold standard for BRD diagnosis, six reference diagnosis methods were used to define an animal as BRD or non-BRD. Sensitivity (Se) and specificity (Sp) were used to estimate diagnostic accuracy (Acc). Blood metabolomics demonstrated a high accuracy at diagnosing BRD when using visual signs of BRD (Acc = 0.85), however was less accurate at diagnosing BRD using rectal temperature (Acc = 0.65), lung auscultation score (Acc = 0.61) and lung lesions at slaughter as reference diagnosis methods (Acc = 0.71). Phenylalanine, lactate, hydroxybutyrate, tyrosine, citrate and leucine were identified as metabolites of importance in classifying animals as BRD or non-BRD. The blood metabolome classified BRD and non-BRD animals with high accuracy and shows potential for use as a BRD diagnosis tool.
Publisher
Springer Science and Business Media LLC
Reference57 articles.
1. Cusack, P. M. V., McMeniman, N. & Lean, I. J. The medicine and epidemiology of bovine respiratory disease in feedlots. Australian Veterinary Journal 81, 480–487, https://doi.org/10.1111/j.1751-0813.2003.tb13367.x (2003).
2. Baptista, A. L. et al. Bovine respiratory disease complex associated mortality and morbidity rates in feedlot cattle from southeastern Brazil. Journal of Infection in Developing Countries 11, 791–799, https://doi.org/10.3855/jidc.9296 (2017).
3. Smith, R. A. Impact of disease on feedlot performance: a review. Journal of Animal Science 76, 272, https://doi.org/10.2527/1998.761272x (1998).
4. Fulton, R. W. & Confer, A. W. Laboratory test descriptions for bovine respiratory disease diagnosis and their strengths and weaknesses: gold standards for diagnosis, do they exist? The Canadian veterinary journal = La revue vétérinaire canadienne 53, 754 (2012).
5. Wolfger, B., Timsit, E., White, B. J. & Orsel, K. A Systematic Review of Bovine Respiratory Disease Diagnosis Focused on Diagnostic Confirmation, Early Detection, and Prediction of Unfavorable Outcomes in Feedlot Cattle. The Veterinary clinics of North America. Food animal practice 31, 351–365, https://doi.org/10.1016/j.cvfa.2015.05.005 (2015).
Cited by
41 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献