A high-throughput imaging and nuclear segmentation analysis protocol for cleared 3D culture models-Reference-Cited by-同舟云学术

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A high-throughput imaging and nuclear segmentation analysis protocol for cleared 3D culture models

Published:2018-07-24 Issue:1 Volume:8 Page:
ISSN:2045-2322
Container-title:Scientific Reports
language:en
Short-container-title:Sci Rep

Author:

Boutin Molly E.,Voss Ty C.,Titus Steven A.,Cruz-Gutierrez Kennie,Michael Sam,Ferrer Marc

Funder

NIH Intramural Research Program; Cures Acceleration Network, NCATS, NIH

Publisher

Springer Science and Business Media LLC

Subject

Multidisciplinary

Link

http://www.nature.com/articles/s41598-018-29169-0.pdf

Reference37 articles.

1. Smietana, K., Siatkowski, M. & Møller, M. Trends in clinical success rates. Nat. Rev. Drug Discov. 15, 379–380 (2016).

2. Friedrich, J., Seidel, C., Ebner, R. & Kunz-Schughart, L. A. Spheroid-based drug screen: considerations and practical approach. Nat. Protoc. 4, 309–324 (2009).

3. Abbott, A. Biology’s new dimension. Nature 424, 870–872 (2003).

4. Birgersdotter, A., Sandberg, R. & Ernberg, I. Gene expression perturbation in vitro-A growing case for three-dimensional (3D) culture systems. Semin. Cancer Biol. 15, 405–12 (2005).

5. Edmondson, R., Broglie, J. J., Adcock, A. F. & Yang, L. Three-Dimensional Cell Culture Systems and Their Applications in Drug Discovery and Cell-Based Biosensors. Assay Drug Dev. Technol. 12, 207–218 (2014).

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