Author:
de Sousa Fátima Regina Nunes,de Sousa Ferreira Vanessa Costa,da Silva Martins Conceição,Dantas Hugo Victor,de Sousa Frederico Barbosa,Girão-Carmona Virgínia Cláudia Carneiro,Goes Paula,de Castro Brito Gerly Anne,de Carvalho Leitão Renata Ferreira
Abstract
AbstractZoledronic acid (ZA) is often prescribed for osteoporosis or resorptive metabolic bone disease. This study aims to evaluate the effect of ZA on orthodontic tooth movement (OTM) and root and bone resorption and its repercussion on root, periodontal ligament and alveolar bone tissues. The experimental group consisted of 72 Wistar rats divided in four subgroups: Naive, Saline and Zoledronic Acid groups at the concentration of 0.2 mg/kg [ZA (0.2)] or 1.0 mg/kg [ZA (1.0)]. The animals were subjected to i.v (dorsal penile vein) administrations of ZA or saline solution, on days 0, 7, 14 and 42. Under anesthesia, NiTi springs were installed in the first left maxillary molar with 50gf allowing the OTM, except for the negative control group (N) for mesial movement of the left first maxillary teeth. The animals were sacrificed and maxillae were removed for macroscopic and histopathological analyzes, scanning electron microscopy, computerized microtomography and confocal microscopy. Treatment with ZA decreased the OTM and the number of osteoclasts and loss of alveolar bone when compared to the naive and saline groups. Reduction of radicular resorption, increased necrotic areas and reduced vascularization in the periodontal ligament were observed in the ZA groups. ZA interferes with OTM and presents anti-resorptive effects on bone and dental tissues associated with a decreased vascularization, without osteonecrosis.
Publisher
Springer Science and Business Media LLC
Reference40 articles.
1. Florencio-Silva, R., Sasso, G.R.D.S., Sasso-Cerri, E., Simões, M.J. & Cerri, P.S. Biology of bone tissue: Structure, function, and factors that influence bone cells. BioMed. Res. Int. 2015, 721742, https://doi.org/10.1155/2015/421746 (2015).
2. Lu, H. et al. Effects of subchronic exposure to lead acetate and cadmium chloride on rat’s bone: Ca and Pi contents, bone density, and histopathological evaluation. Int. J. Clin. Exp. Pathol. 7(2), 640–647 (2014).
3. Field, R. A. Ash and calcium as measures of bone in meat and bone mixtures. Meat Sci. 55(3), 255–264 (2000).
4. Jiang, C. et al. Osteoimmunology in orthodontic tooth movement. Oral Dis. 21(6), 694–704 (2015).
5. Alphy, A., Kannan, T., Norazmi, M. & Nurul, A. Interleukin-17A promotes osteogenic differentiation by increasing OPG/RANKL ratio in stem cells from human exfoliated deciduous teeth. J. Tissue Eng. Regen. Med. 12(8), 1856–1866 (2018).
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