Author:
Himaya S. W. A.,Jin Ai-Hua,Hamilton Brett,Rai Subash K.,Alewood Paul,Lewis Richard J.
Abstract
AbstractThe venom duct origins of predatory and defensive venoms has not been studied for hook-and-line fish hunting cone snails despite the pharmacological importance of their venoms. To better understand the biochemistry and evolution of injected predatory and defensive venoms, we compared distal, central and proximal venom duct sections across three specimens of C. striatus (Pionoconus) using proteomic and transcriptomic approaches. A total of 370 conotoxin precursors were identified from the whole venom duct transcriptome. Milked defensive venom was enriched with a potent cocktail of proximally expressed inhibitory α-, ω- and μ-conotoxins compared to milked predatory venom. In contrast, excitatory κA-conotoxins dominated both the predatory and defensive venoms despite their distal expression, suggesting this class of conotoxin can be selectively expressed from the same duct segment in response to either a predatory or defensive stimuli. Given the high abundance of κA-conotoxins in the Pionoconus clade, we hypothesise that the κA-conotoxins have evolved through adaptive evolution following their repurposing from ancestral inhibitory A superfamily conotoxins to facilitate the dietary shift to fish hunting and species radiation in this clade.
Publisher
Springer Science and Business Media LLC
Reference50 articles.
1. MolluscaBase (2020). MolluscaBase. Conus Linnaeus, 1758. Accessed through: World Register of Marine Species at: http://www.marinespecies.org/aphia.php?p=taxdetails&id=137813. Accessed 19 Nov 2020.
2. Lewis, R. J., Dutertre, S., Vetter, I. & Christie, M. J. Conus venom peptide pharmacology. Pharm. Rev. 64(2), 259–298 (2012).
3. Jin, A. H. et al. Conotoxins: Chemistry and biology. Chem. Rev. 119(21), 11510–11549 (2019).
4. Dutertre, S. et al. Evolution of separate predation-and defence-evoked venoms in carnivorous cone snails. Nat. Commun. 5(1), 1–9 (2014).
5. Jin, A. H. et al. Transcriptome and proteome of Conus planorbis identify the nicotinic receptors as primary target for the defensive venom. Proteomics 15(23–24), 4030–4040 (2015).
Cited by
6 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献