Author:
Tritten Lucienne,Gillis-Germitsch Nina,Kockmann Tobias,Schnyder Manuela
Abstract
AbstractBlood contains hundreds of proteins, reflecting ongoing cellular processes and immune reactions. Infections with the blood-dwelling cardiopulmonary nematode Angiostrongylus vasorum in dogs manifest with a broad spectrum of clinical signs including respiratory distress, bleeding diathesis and neurological signs, and are associated with a perturbed blood protein profile in dogs. However, current knowledge does not completely explain the observed pathologies induced by A. vasorum infections, including bleeding disorders. Using sera from experimentally infected dogs, dog serum proteome was analysed by quantitative mass spectrometry methods over several time points before and after inoculation. Following computational analysis, we identified 139 up- and downregulated proteins after infection (log2 ratio cut-off ≥ 1.0; q-value ≤ 0.05). Among upregulated proteins were chitinase 3-like 1 and pulmonary surfactant-associated protein B (log2 fold-changes ≥ 5). Pathway enrichment revealed the complement (especially the lectin pathway) and coagulation cascades as significantly affected upon analysis of downregulated proteins. Among them were mannan-binding lectin serine peptidases, ficolin, and coagulation factor XIII-B. These results bring new elements towards understanding the underlying pathomechanisms of bleeding diatheses observed in some A. vasorum-infected dogs.
Funder
Forschungskredit of the University of Zurich
Privatdozierende Foundation
Publisher
Springer Science and Business Media LLC
Reference63 articles.
1. Rosen, L., Ash, L. R. & Wallace, G. D. Life history of the canine lungworm Angiostrongylus vasorum (Baillet). Am. J. Vet. Res. 31, 131–143 (1970).
2. Elsheikha, H. M., Holmes, S. A., Wright, I., Morgan, E. R. & Lacher, D. W. Recent advances in the epidemiology, clinical and diagnostic features, and control of canine cardio-pulmonary angiostrongylosis. Vet. Res. 45, 92 (2014).
3. Guilhon, J. & Cens, B. Angiostrongylus vasorum (Baillet, 1866) Etude biologique et morphologique. Ann. Parasitol. Hum. Comparée 48, 567–596 (1973).
4. Koch, J. & Willesen, J. L. Canine pulmonary angiostrongylosis: An update. Vet. J. Lond. Engl. 1997(179), 348–359 (2009).
5. Schnyder, M. et al. Clinical, laboratory and pathological findings in dogs experimentally infected with Angiostrongylus vasorum. Parasitol. Res. 107, 1471–1480 (2010).
Cited by
11 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献