Author:
Santovito Alfredo,Gendusa Claudio,Cervella Piero,Traversi Deborah
Abstract
AbstractUrban air pollution represents a global problem, since everyday many mutagenic and carcinogens compounds are emitted into the atmosphere, with consequent adverse health effects on humans and biota. Specifically, particulate matter air pollution was associated with increased risks in human mortality and morbidity. In this paper, we analyse the genomic effects on human lymphocytes of different concentrations of annual Turin PM2.5 extract by an in vitro micronuclei assay. Samplings were collected from an urban meteorological-chemical station positioned in Turin (Italy), one of the most polluted cities in Europe. PM2.5 sampled on filters was used for organic extraction in monthly pools and successively aggregated to produce a mixture representative for a full year PM2.5 collection. Lymphocytes were exposed to four concentrations of PM2.5: 5, 10, 15 and 20 μg/mL and micronuclei, nucleoplasmic bridges and nuclear buds were scored. With respect to controls, PM2.5 significantly increased the frequencies of all analysed biomarkers at all tested concentrations, whereas the CBPI index was significantly reduced only at the concentration of 20 μg/mL. Such in vitro effects can both to stimulate local authorities to adopt efficient measures for air pollution mitigation and to improve human monitoring to detect early precancer lesions.
Publisher
Springer Science and Business Media LLC
Reference52 articles.
1. Pope, C. A. III et al. Lung cancer, cardiopulmonary mortality, and long-term exposure to fine particulate air pollution. JAMA 287, 1132–1141 (2002).
2. Sørensen, M. et al. Personal PM2.5 exposure and markers of oxidative stress in blood. Env. Health Perspect. 111, 161–166 (2003a).
3. Sørensen, M. et al. Linking exposure to environmental pollutants with biological effects. Mutat. Res. 544, 255–271 (2003b).
4. Silva, R. A. et al. Global premature mortality due to anthropogenic outdoor air pollution and the contribution of past climate change. Env. Res. Lett. 8, 1–11 (2013).
5. World Health Organization (WHO) Ambient air pollution: A global assessment of exposure and burden of disease. Geneva, Switzerland: WHO. Available online at, http://apps.who.int/iris/bitstream/handle/10665/250141/9789241511353-eng.pdf;jsessionid=6B835E56443CA533658F0AC0A743D854?sequence=1, Accessed on 03-05-2019 (2016).
Cited by
14 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献