Author:
Nicholas Dequina A.,Knight Vashti S.,Tonsfeldt Karen J.,Terasaka Tomohiro,Molinar-Inglis Olivia,Stephens Shannon B. Z.,Trejo JoAnn,Kauffman Alexander S.,Mellon Pamela L.,Lawson Mark A.
Abstract
AbstractThe mechanisms mediating suppression of reproduction in response to decreased nutrient availability remain undefined, with studies suggesting regulation occurs within the hypothalamus, pituitary, or gonads. By manipulating glucose utilization and GLUT1 expression in a pituitary gonadotrope cell model and in primary gonadotropes, we show GLUT1-dependent stimulation of glycolysis, but not mitochondrial respiration, by the reproductive neuropeptide GnRH. GnRH stimulation increases gonadotrope GLUT1 expression and translocation to the extracellular membrane. Maximal secretion of the gonadotropin Luteinizing Hormone is supported by GLUT1 expression and activity, and GnRH-induced glycolysis is recapitulated in primary gonadotropes. GLUT1 expression increases in vivo during the GnRH-induced ovulatory LH surge and correlates with GnRHR. We conclude that the gonadotropes of the anterior pituitary sense glucose availability and integrate this status with input from the hypothalamus via GnRH receptor signaling to regulate reproductive hormone synthesis and secretion.
Funder
National Institutes of Health
University of California
Publisher
Springer Science and Business Media LLC
Reference68 articles.
1. Aladashvili-Chikvaidze, N., Kristesashvili, J. & Gegechkori, M. Types of reproductive disorders in underweight and overweight young females and correlations of respective hormonal changes with BMI. Iran. J. Reprod. Med. 13, 135–140 (2015).
2. Chikvaidze, N., Kristesashvili, J. & Gegechkori, M. Peculiarities of sexual development and reproductive function in young women with childhood onset weight problems. Georgian Med. News 235, 11–16 (2014).
3. Tropp, J. & Markus, E. J. Effects of mild food deprivation on the estrous cycle of rats. Physiol. Behav. 73, 553–559. https://doi.org/10.1016/s0031-9384(01)00487-5 (2001).
4. Kumar, S. & Kaur, G. Intermittent fasting dietary restriction regimen negatively influences reproduction in young rats: A study of hypothalamo-hypophysial-gonadal axis. PLoS ONE 8, e52416. https://doi.org/10.1371/journal.pone.0052416 (2013).
5. Nagatani, S. et al. Reduction of glucose availability suppresses pulsatile luteinizing hormone release in female and male rats. Endocrinology 137, 1166–1170. https://doi.org/10.1210/endo.137.4.8625885 (1996).