Npro fusion technology to produce proteins with authentic N termini in E. coli

Author:

Achmüller Clemens,Kaar Waltraud,Ahrer Karin,Wechner Philipp,Hahn Rainer,Werther Florian,Schmidinger Hannes,Cserjan-Puschmann Monika,Clementschitsch Franz,Striedner Gerald,Bayer Karl,Jungbauer Alois,Auer Bernhard

Publisher

Springer Science and Business Media LLC

Subject

Cell Biology,Molecular Biology,Biochemistry,Biotechnology

Reference29 articles.

1. Georgiou, G. & Valax, P. Isolating inclusion bodies from bacteria. Methods Enzymol. 309, 48–58 (1999).

2. Jungbauer, A. & Kaar, W. Current status of technical protein refolding. J. Biotechnol. 128, 587–596 (2007).

3. Sandman, K., Grayling, R.A. & Reeve, J.N. Improved N-terminal processing of recombinant proteins synthesized in Escherichia coli. Bio/Technology 13, 504–506 (1995).

4. Adams, J.M. On the release of the formyl group from nascent protein. J. Mol. Biol. 33, 571–589 (1968).

5. Yem, A.W., Richard, K.A., Staite, N.D. & Deibel, M.R., Jr. Resolution and biological properties of three N-terminal analogues of recombinant human interleukin-1β. Lymphokine Res. 7, 85–92 (1988).

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