Integrating genomic and multiomic data for Angelica sinensis provides insights into the evolution and biosynthesis of pharmaceutically bioactive compounds

Author:

Li Shiming,Chiu Tsan-YuORCID,Jin Xin,Cao Dong,Xu MengORCID,Zhu Mingzhi,Zhou Qi,Liu Chun,Zong Yuan,Wang Shujie,Yu Kang,Zhang Feng,Bai Mingzhou,Liu Guangrui,Liang Yunlong,Zhang Chi,Simonsen Henrik Toft,Zhao JianORCID,Liu BaolongORCID,Zhao ShancenORCID

Abstract

AbstractAngelica sinensis roots (Angelica roots) are rich in many bioactive compounds, including phthalides, coumarins, lignans, and terpenoids. However, the molecular bases for their biosynthesis are still poorly understood. Here, an improved chromosome-scale genome for A. sinensis var. Qinggui1 is reported, with a size of 2.16 Gb, contig N50 of 4.96 Mb and scaffold N50 of 198.27 Mb, covering 99.8% of the estimated genome. Additionally, by integrating genome sequencing, metabolomic profiling, and transcriptome analysis of normally growing and early-flowering Angelica roots that exhibit dramatically different metabolite profiles, the pathways and critical metabolic genes for the biosynthesis of these major bioactive components in Angelica roots have been deciphered. Multiomic analyses have also revealed the evolution and regulation of key metabolic genes for the biosynthesis of pharmaceutically bioactive components; in particular, TPSs for terpenoid volatiles, ACCs for malonyl CoA, PKSs for phthalide, and PTs for coumarin biosynthesis were expanded in the A. sinensis genome. These findings provide new insights into the biosynthesis of pharmaceutically important compounds in Angelica roots for exploration of synthetic biology and genetic improvement of herbal quality.

Funder

QingHai Department of Science and Technology

Shenzhen Science and Technology Innovation Commission

special funds for Science, Technology, Innovation and Industrial Development of Shenzhen Dapeng New District

Publisher

Springer Science and Business Media LLC

Subject

General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology,Medicine (miscellaneous)

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