Metagenomic data from cerebrospinal fluid permits tracing the origin and spread of Neisseria meningitidis CC4821 in China

Author:

Chen HongbinORCID,Li Mei,Tu Shangyu,Zhang Xiaoyang,Wang Xiaojuan,Zhang Yawei,Zhao Chunjiang,Guo Yinghui,Wang HuiORCID

Abstract

AbstractMetagenomic next-generation sequencing (mNGS) is useful for difficult to cultivate pathogens. Here, we use cerebrospinal fluid mNGS to diagnose invasive meningococcal disease. The complete genome sequences of Neisseria meningitidis were assembled using N. meningitidis of ST4821-serotype C isolated from four patients. To investigate the phylogeny, 165 CC4821 N. meningitidis genomes from 1972 to 2017 were also included. The core genome accumulated variation at a rate of 4.84×10−8 substitutions/nucleotide site/year. CC4821 differentiated into four sub-lineages during evolution (A, B, C, and D). While evolving from sub-lineage A (early stage) to sub-lineage D (late stage), the ST and CC4821 serotype converged into the ST4821-serotype C clone. Most strains of sub-lineage D were isolated from invasive meningococcal disease, with increasing resistance to quinolones. Phylogeographic analysis suggests that CC4821 has spread across 14 countries. Thus, the selective pressure of quinolones may cause CC4821 to converge evolutionarily, making it more invasive and facilitating its spread.

Funder

National Science and Technology Major Project

Publisher

Springer Science and Business Media LLC

Subject

General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology,Medicine (miscellaneous)

Reference44 articles.

1. Hill, D. J., Griffiths, N. J., Borodina, E. & Virji, M. Cellular and molecular biology of Neisseria meningitidis colonization and invasive disease. Clin. Sci. 118, 547–564 (2010).

2. Johri, S., Gorthi, S. P. & Anand, A. C. Meningococcal meningitis. Med. J. Armed Forces India 61, 369–374 (2005).

3. Winstanley, F. P., Blackwell, C. C. & Weir, D. M. Factors influencing host susceptibility to meningococcal disease. Biomed. Pharmacother. 39, 167–170 (1985).

4. Rosenstein, N. E. et al. Meningococcal disease. N. Engl. J. Med. 344, 1378–1388 (2001).

5. Cohn, A. C. et al. Prevention and control of meningococcal disease: recommendations of the Advisory Committee on Immunization Practices (ACIP). MMWR Recomm. Rep. 62, 1–28 (2013).

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