Optimized protocols for sperm cryopreservation and in vitro fertilization in the rat
Author:
Funder
A research grant from Kyudo Co. Ltd.
Publisher
Springer Science and Business Media LLC
Subject
General Veterinary,Animal Science and Zoology
Link
https://www.nature.com/articles/s41684-022-01053-5.pdf
Reference65 articles.
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3. Li, D. et al. Heritable gene targeting in the mouse and rat using a CRISPR–Cas system. Nat. Biotechnol. 31, 681–683 (2013).
4. Li, W., Teng, F., Li, T. & Zhou, Q. Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR–Cas systems. Nat. Biotechnol. 31, 684–686 (2013).
5. Yoshimi, K. et al. ssODN-mediated knock-in with CRISPR–Cas for large genomic regions in zygotes. Nat. Commun. 7, 10431 (2016).
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