Avoiding artefacts when counting polymerized actin in live cells with LifeAct fused to fluorescent proteins
Author:
Publisher
Springer Science and Business Media LLC
Subject
Cell Biology
Link
http://www.nature.com/articles/ncb3351.pdf
Reference42 articles.
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3. Cooper, J. A., Walker, S. B. & Pollard, T. D. Pyrene actin: documentation of the validity of a sensitive assay for actin polymerization. J. Muscle Res. Cell Motil. 4, 253–262 (1983).
4. Amann, K. J. & Pollard, T. D. Direct real-time observation of actin filament branching mediated by Arp2/3 complex using total internal reflection fluorescence microscopy. Proc. Natl Acad. Sci. USA 98, 15009–15013 (2001).
5. Wu, J. Q. & Pollard, T. D. Counting cytokinesis proteins globally and locally in fission yeast. Science 310, 310–314 (2005).
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