Abstract
AbstractConnectomes of human cortical gray matter require high-contrast homogeneously stained samples sized at least 2 mm on a side, and a mouse whole-brain connectome requires samples sized at least 5–10 mm on a side. Here we report en bloc staining and embedding protocols for these and other applications, removing a key obstacle for connectomic analyses at the mammalian whole-brain level.
Publisher
Springer Science and Business Media LLC
Subject
Cell Biology,Molecular Biology,Biochemistry,Biotechnology
Reference30 articles.
1. Karnovsky, M. J. Use of ferrocyanide-reduced osmium tetroxide in electron microscopy. In 11th Annual Meeting, the American Society for Cell Biology (ASCB, 1971; 146).
2. de Bruijn, W. C. Glycogen, its chemistry and morphologic appearance in the electron microscope I. A modified OsO4 fixative which selectively contrast glycogen. J. Ultrastruct. Res. 42, 29–50 (1973).
3. Willingham, C. & Rutherford, A. Use of osmium-thiocarbohydrazide-osmium and ferrocyanide-reduced methods to enhance membrane contrast and preservation in cultured cells. J. Histochem. Cytochem. 32, 455–460 (1984).
4. Briggman, K. L., Helmstaedter, M. & Denk, W. Wiring specificity in the direction-selectivity circuit of the retina. Nature 471, 183–190 (2011).
5. Holcomb, P. S. et al. Synaptic inputs compete during rapid formation of the calyx of Held: a new model system for neural development. J. Neurosci. 33, 12954–12969 (2013).
Cited by
11 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献