Optimization of a Method for Ficin Immobilization Using Glutaraldehyde-Reference-Cited by-同舟云学术

Optimization of a Method for Ficin Immobilization Using Glutaraldehyde

Published:2020 Issue:5 Volume:36 Page:81-88
ISSN:2500-2341
Container-title:Biotekhnologiya
language:ru
Short-container-title:Biiotehnologiâ (Mosk.)

Author:

Holyavka M.G.¹,Artyukhov V.G.¹

Affiliation:

1. Voronezh State University, Voronezh, 394018, Russia

Abstract

Ficin was covalently immobilized on an acid-soluble matrix of medium (200 kDa) and high molecular weight (350 kDa) chitosans. The enzyme molecules were immobilized in a protein film and copolymerized with glutaraldehyde. The optimal ratio of protein content, total activity and specific activity was observed as a result of ficin covalent immobilization on a matrix of medium-molecular chitosan with 15% glutaraldehyde and high-molecular chitosan with 10% glutaraldehyde. The obtained biocatalysts are promising for industrial applications. ficin, chitosan, glutaraldehyde, covalent immobilization. This work was financially supported by a grant from the President of the Russian Federation for state support to young Russian scientists-doctors of sciences MD-1982.2020.4. Agreement 075-15-2020-325).

Publisher

National Research Center Kurchatov Institute

Subject

Ecology,Applied Microbiology and Biotechnology,Biotechnology

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