Cryopreservation and Rapid Recovery of Differentiated Intestinal Epithelial Barrier Cells at Complex Transwell Interfaces Is Enabled by Chemically Induced Ice Nucleation

Author:

Bissoyi Akalabya12,Gao Yanan34,Tomás Ruben M. F.5,Kinney Nina L. H.36ORCID,Whale Thomas F.37ORCID,Guo Qiongyu4ORCID,Gibson Matthew I.3812ORCID

Affiliation:

1. Department of Chemistry, University of Manchester, Oxford Road, Manchester M13 9PL, U.K.

2. Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester M1 7DN, U.K.

3. Department of Chemistry, University of Warwick, Coventry CV4 7AL, U.K.

4. Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China

5. Cryologyx Ltd, Venture Centre, University of Warwick Science Park, Coventry CV4 7EZ, U.K.

6. Royal Botanic Gardens Kew, Ardingly, West Sussex RH17 6TN, U.K.

7. School of Earth and Environment, University of Leeds, Leeds LS2 9JT, U.K.

8. Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Coventry CV4 7AL, U.K.

Funder

Royal Society

Leverhulme Trust

Innovate UK

Natural Environment Research Council

Southern University of Science and Technology

National Natural Science Foundation of China

H2020 European Research Council

Biotechnology and Biological Sciences Research Council

University of Warwick

CryoLogyx Ltd.

Cytivia

Publisher

American Chemical Society (ACS)

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