Shotgun Kinetic Target-Guided Synthesis Approach Enables the Discovery of Small-Molecule Inhibitors against Pathogenic Free-Living Amoeba Glucokinases

Author:

Kassu Mintesinot1,Parvatkar Prakash T.1,Milanes Jillian2,Monaghan Neil P.2,Kim Chungsik1,Dowgiallo Matthew1,Zhao Yingzhao1,Asakawa Ami H.3,Huang Lili1,Wagner Alicia1,Miller Brandon1,Carter Karissa1,Barrett Kayleigh F.4,Tillery Logan M.4,Barrett Lynn K.4,Phan Isabelle Q.5ORCID,Subramanian Sandhya5,Myler Peter J.5,Van Voorhis Wesley C.4ORCID,Leahy James W.6ORCID,Rice Christopher A.78910ORCID,Kyle Dennis E.10,Morris James2ORCID,Manetsch Roman131112ORCID

Affiliation:

1. Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts 02115, United States

2. Eukaryotic Pathogens Innovation Center, Department of Genetics and Biochemistry, Clemson University, Clemson, South Carolina 29634, United States

3. Department of Pharmaceutical Sciences, Northeastern University, Boston, Massachusetts 02115, United States

4. Center for Emerging and Re-emerging Infectious Diseases (CERID), Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington School of Medicine, Seattle, Washington 98109, United States

5. Center for Global Infectious Diseases Research, Seattle Children’s Research Center, Seattle, Washington 98109, United States

6. Department of Chemistry, University of South Florida, Tampa, Florida 33620, United States

7. Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, Indiana 47907, United States

8. Purdue Institute for Drug Discovery (PIDD), Purdue University, West Lafayette, Indiana 47907, United States

9. Purdue Institute of Inflammation, Immunology and Infectious Disease (PI4D), Purdue University, West Lafayette, Indiana 47907, United States

10. Department of Cellular Biology, University of Georgia, Athens, Georgia 30602, United States

11. Center for Drug Discovery, Northeastern University, Boston, Massachusetts 02115, United States

12. Barnett Institute of Chemical and Biological Analysis, Northeastern University, Boston, Massachusetts 02115, United States

Funder

U.S. Department of Health and Human Services

Publisher

American Chemical Society (ACS)

Subject

Infectious Diseases

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