A Novel Confocal Scanning Protein–Protein Interaction Assay (PPI-CONA) Reveals Exceptional Selectivity and Specificity of CC0651, a Small Molecule Binding Enhancer of the Weak Interaction between the E2 Ubiquitin-Conjugating Enzyme CDC34A and Ubiquitin-Reference-Cited by-同舟云学术

A Novel Confocal Scanning Protein–Protein Interaction Assay (PPI-CONA) Reveals Exceptional Selectivity and Specificity of CC0651, a Small Molecule Binding Enhancer of the Weak Interaction between the E2 Ubiquitin-Conjugating Enzyme CDC34A and Ubiquitin

Published:2024-08-21 Issue: Volume: Page:
ISSN:1043-1802
Container-title:Bioconjugate Chemistry
language:en
Short-container-title:Bioconjugate Chem.

Author:

Koszela Joanna¹^ORCID,Pham Nhan T.²³^ORCID,Shave Steven²⁴^ORCID,St-Cyr Daniel⁵⁶^ORCID,Ceccarelli Derek F.⁷,Orlicky Steven⁷,Marinier Anne⁶,Sicheri Frank⁷^ORCID,Tyers Mike⁶⁸,Auer Manfred²^ORCID

Affiliation:

1. School of Molecular Biosciences, University of Glasgow, Glasgow G12 8QQ, U.K.

2. School of Biological Sciences, University of Edinburgh, Edinburgh, Scotland EH9 3BF, U.K.

3. College of Medicine and Veterinary Medicine, Institute for Regeneration and Repair, University of Edinburgh, 4-5 Little France Drive, Edinburgh EH16 4UU, U.K.

4. Edinburgh Cancer Research, Cancer Research UK Scotland Centre, Institute of Genetics and Cancer, University of Edinburgh, Crewe Road South, Edinburgh EH4 2XR, U.K.

5. X-Chem Inc., Montréal, Québec H4S 1Z9, Canada

6. Institute for Research in Immunology and Cancer, University of Montreal, Montreal, Québec H3T 1J4, Canada

7. Centre for Systems Biology, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada

8. Program in Molecular Medicine, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada

Funder

Canadian Institutes of Health Research

Medical Research Council

Wellcome Trust

Scottish Universities Life Sciences Alliance

Publisher

American Chemical Society (ACS)

Link

https://pubs.acs.org/doi/pdf/10.1021/acs.bioconjchem.4c00345

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