Testing of experimental brucellosis antigens in a stimulated cell test with nitroblue tetrazolium

Abstract

The results of testing experimental samples of brucellosis antigens as an operator when performing a stimulated activated cell test with nitroblue tetrazolium in vitro are presented. Experimental samples of brucellosis preparations from Brucella strains with different antigenic structures were used in laboratory conditions. Experimental studies were carried out on guinea pigs immunized with Brucella’s R-strain. The activity and specificity of additional samples and the optimal concentration providing the stimulating potential of neutrophils in the blood of immunized guinea pigs were determined. At the first stages of the study, it was noted that Brucella disintegrates. Corpuscular antigens in concentrations were 50 and 100 μg/ml, and this did not cause nonspecific activation of neutrophil granulocytes in the NBT test with the blood of intact animals. Subsequently, we studied the immune response dynamics of immunized guinea pigs in the NBT test using experimental samples of brucellosis antigens in optimal concentrations. The biomaterial selection for research was carried out before immunization and on the 7th, 14th, 21st, 28th, 42nd, 55th, 69th, and 125th day after vaccination. The stimulation coefficient was calculated as the ratio of the induced level of cellular activity to the spontaneous one. It was found that the maximum level of specific activation of neutrophils in the NBT test is characteristic exclusively on the 28th day, corresponding to the peak synthesis of agglutinating and complement-fixing antibodies in serological reactions. The data obtained from the experiment allow us to conclude that it is possible to use experimental samples of brucellosis antigens as a stimulant when performing an NBT test with the Foundation for determining the immune control of cellular restructuring of the body during the formation of the response.