Abstract
a-Bungarotoxin is one of a class of proteins, isolated from snake venoms, which antagonize the action of acetylcholine at vertebrate neuromuscular junctions and ‘electroplaques' of electric fish. a-Bungarotoxin blocks acetylcholine action irreversibly and may be labelled with either
125
I or
3
H. This irreversible binding is used as the basis of an
in vitro
assay for acetylcholine receptors, whether in intact tissue, membrane fragments or solubilized preparations. Acetylcholine receptors from
Torpedo
and denervated skeletal muscle have been solubilized and substantially purified using affinity chromatography. The distribution of acetylcholine receptors in several tissues has been determined, and an auto-immune response, induced by injection of purified
Torpedo
receptors, has been studied.
Subject
Industrial and Manufacturing Engineering,General Agricultural and Biological Sciences,General Business, Management and Accounting,Materials Science (miscellaneous),Business and International Management
Cited by
47 articles.
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