Preparation of Nano Electrospinning Covered Stent and Its Application in the Treatment of Intracranial Aneurysm with CT Diagnosis

Abstract

In the process of tissue regeneration or organ reconstruction, tissue engineering scaffolds provide strong structural support for the function of seed cells, which enables tissue engineering technology to be implemented. In the treatment of intracranial aneurysm, from the perspective of endovascular treatment, the emulsion electro-spinning method is used and the lactic acid caprolactone copolymer is taken as the matrix material to design a kind of vascular arterial stent with heparin loaded nanofibers as a film covering layer. SEM confirms that the stent has a good nanofiber structure, and TEM suggests that the core layer containing fluorescein is evenly distributed around the stent. Fourier transform infrared reflection (FTIR) shows that heparin is successfully loaded into the stent. WCA test indicates that heparin loading can improve the hydrophilicity of stent. In vitro biocompatibility/hemocompatibility test shows that the stent has good compatibility, but inhibits the proliferation of L929 and PIEC cells. With the stent as the film covering layer, the heparin loaded nanofiber stent for small vascular aneurysms successfully prepared has achieved good results in the treatment of intracranial aneurysm. CT imaging of patients treated with vascular arterial stent shows that heparin in tumor site degrades with time, which makes CT signal in mice change with time. At the same time, aneurysms gradually disappear with the implantation of covered stent. After implantation 30 d, the blood vessels of the implanted site are unobstructed and no thrombus appears.