Effect of Preparation of β2M Nano Antibody Adsorbent for Blood Purification on Dialysis Complications in Patients with Renal Failure

Abstract

In order to purify blood and adsorb β2M, a highly selective immunosorbent biomaterial based on nano antibody was designed. First of all, nanoantibodies against β2M in patients with renal failure are screened by phage display library technology. After 4 rounds of screening, 12 kinds of monoclonal phage samples are compared by ELISA, and the highest affinity sequence A53 is obtained. Then, the nano antibody A53 is transferred into two kinds of host bacteria respectively, and the host E. coli Shuffle T7 is selected, and then purified by nickel column affinity, thus obtaining high purity nano antibody A53S. Five kinds of nano antibody adsorbents are prepared by using A53S as a component of adsorbent. The results show that the effect of D series adsorbent with ɛ-poly-L-lysine as spacer is better, but the ligand density should be adjusted reasonably to ensure the activity of nanoantibody protein. When the loading concentration of β2M is set to 1.8 mg/ml, adsorbent D-1 can adsorb 11.78 mg/ml of dynamic adsorption capacity of β2M. This index is significantly higher than the dynamic adsorption capacity of plasma system under the same conditions (P < 0.05), which proves that the nano antibody adsorbent biomaterial can effectively adsorb β2M, thus reducing the probability of dialysis complications.