Bacterial Isolation by Adsorption on Graphene Oxide from Large Volume Sample

Abstract

Graphene oxide (GO) is a well-known two-dimensional nanomaterial with broad applications in various fields. In particular, the functional groups of GO has demonstrated significance in the molecular binding interactions. GO is normally coated on a solid surface as it is difficult to handle due to its nano-scaled size. Therefore, chemical properties of surface-coated GO depend on the morphological structure of GO on the surface and the operating conditions during the coating process. Isolation of bacteria from environmental samples such as river and pond water is important for increasing the analytical sensitivity of sensor devices. The main issue in isolation of bacteria from an environmental sample is adsorption capacity per unit time. However, increasing the velocity of water sample to elevate the process rate induces high shear stress on the surface, such that the bacteria adsorption rate on the surface is reduced. In this study, we investigated the morphological and chemical properties of sonicated GO and GO-coated surface by atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS). The sonicated GO-coated beads were successfully used for concentrating bacteria from a large-volume sample as opposed to the conventional methods. It can be concluded that, GO-coated surfaces are prospective platforms for concentrating bacteria from various samples and play a major role in reducing the concentration time.