Effect of miR-200b Liposome Nanoparticles on Chemotherapy Resistance in Rats with Breast Cancer Through Induction of Epidermal Growth Factor Receptor/Extracellular Signal-Regulated Kinase Signal Pathway

Abstract

This study assessed the effect of miR-200b liposome nanoparticles in restraining chemotherapy resistance in rats with breast cancer through induction of epidermal growth factor receptor enzyme-linked immunosorbent assay enzyme-linked immunosorbent assay (EGFR)/extracellular regulated protein kinases (ERK) signal pathway. 50 rats were divided into 4 sets, which included control set, empty carrier set, miR-200b set, and set of miR-200b packaged with liposome nanoparticles. The liposome nanoparticles were prepared and identified, and drug-resistant breast cancer cells were observed and identified. The growth inhibition ratio, miR-200b expression, drug-resistance, growth curve, drug resistance of cells, EGFR and ERK protein expressions were observed. miR-200b expression in the et of miR-200b packaged with liposome nanoparticles was highest, second in the miR-200b set and empty carrier set, and lowest in the control set. The IC50 value in the miR-200b packaged with liposome nanoparticles was highest. The absorbance in the set of miR-200b liposome nanoparticles was lowest. The immunofluorescence (IF) strength of miR-200b in the miR-200b liposome nanoparticles was highest. The EGFR and ERK protein expressions, and levels of pEGFR and p-ERK in the miR-200b liposome nanoparticles set was highest. In conclusion, chemotherapy resistance of breast cancer cells could be restrained by miR-200b liposome nanoparticles through restraining of the EGFR/ERK signal pathway.