Predictive Fuzzy Neural Network Based Effect of Heat Shock Protein on Prognosis of Tumor Cells

Abstract

In this study, a mouse model of pancreatic carcinoma in situ and a subcutaneous model were used to simulate the actual situation of pancreatic carcinoma after operation, Mice inoculated with Mycobacterium tuberculosis heat shock protein 65 (mHSP65) pancreatic cancer tissue-derived lysate (TDL) and pancreatic cancer cellderived lysate (TCL) respectively to prepare pancreatic cancer vaccine, and combined with low dose cyclophosphamide (CCY) to observe its anti-pancreatic cancer effect in tumor-bearing mice. The experiments showed that survival time of tumor-bearing mice can be prolong by the combination of mHSP65-TCL and CY, decrease the tumor-producing rate, inhibit tumor growth and induce long-term immune memory, but the combination of mHSP65-TCL and CY could not show this effect. We further validated the antitumor effect and mechanism of mHSP65-TTL combined with CY in vivo.