Effect of Porous Zirconia Coating on Human Gingival Fibroblasts and Its Mechanism
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Published:2022-04-01
Issue:4
Volume:18
Page:1164-1171
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ISSN:1550-7033
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Container-title:Journal of Biomedical Nanotechnology
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language:en
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Short-container-title:j biomed nanotechnol
Author:
Hu Jiangqi1,
Qie Yue2,
Luo Yu1,
Jiang Qingsong1
Affiliation:
1. Beijing Stomatological Hospital, Capital Medical University, Beijing, 100050, China
2. Beijing United Family Hospital, Beijing, 100015, China
Abstract
Gingival fibroblasts play an important role in the constitution of soft tissue attachment. This study aims to investigate whether porous zirconia coating has a positive effect on promoting human gingival fibroblast attachment. The porous zirconia coating was loaded on zirconia surface
by the dip coating method, surface morphology and composition were confirmed by scanning electron microscope and energy dispersive spectrometer; Tested the tensile bond strength by universal testing machine; Tested the surface roughness by roughness analyzer; Human gingival fibroblast proliferation,
integrin β1 and F-actin immunofluorescence staining explored the influence of porous zirconia on the adhesion and proliferation of human gingival fibroblast. Zirconia0.2 group showed spherical zirconia particles with diameters of 3–8 μm are distributed on the surface;
The bonding strength of zirconia particle coating group reached 16.1±0.1 MPa, and the surface roughness was 0.715±0.091 μm; In comparison with control group (P < 0.01), the percentage of human gingival fibroblasts adhering to zirconia was markedly higher.
In zirconia group, integrin-β1 and F-actin fluoresced more obvious than in control group. Porous zirconia coating can form a porous structure on the surface and the porous structure can promote the attachment and proliferation of human gingival fibroblast, it will be more beneficial
for soft tissue early sealing.
Publisher
American Scientific Publishers
Subject
Pharmaceutical Science,General Materials Science,Biomedical Engineering,Medicine (miscellaneous),Bioengineering