Antitumor Effect of Co-Loading Natural Active Compound of Okofuran (Usenamine) and Photosensitizer Nano-Liposomes

Abstract

Objective: In this study, we developed a nano-liposome (LIP-RUA) to evaluate the in vitro anti-lung cancer activity. In this regard, nano-liposome co-loaded with photosensitizer (RB), upconverting nanoparticles (UCNPs), and natural active compound usenamine (ACU). Methods: LIP-RUA was obtained by encapsulating ACU/RB/UCNPs by thin film dispersion method. The physicochemical properties were investigated by using an instrument; the efficiency of liposomes producing reactive oxygen species (ROS) was detected by SOSG probe; the uptake of LIP-RUA by A549 lung cancer cells was observed by confocal microscopy. Results: The particle size of the prepared LIP-RUA was about 150 nm, the surface potential was about −12 mV, and the entrapment efficiency of RB and ACU reached 54.5% and 86.5%, respectively. Experimental tasks showed that LIP-RUA could significantly improve the growth inhibitory effect of the drug on lung cancer cells, and the median effective inhibitory concentration (IC50) under laser irradiation was 15.33 μmol/L. Conclusion: LIP-RUA provides a new idea for the combination of photodynamic chemotherapy for the treatment of lung cancer. The liposome platform is expected to enhance the in vivo penetration of photodynamic therapy and the combined effect of photodynamic chemotherapy.