Intra-Epidural Space Injection of OX26-PEGylated Selenium Nanoparticles Enhances Motor Function and Decrease the Risk of Neural Damage in Animal Model of Subarachnoid Hemorrhage
-
Published:2022-05-01
Issue:5
Volume:18
Page:1481-1487
-
ISSN:1550-7033
-
Container-title:Journal of Biomedical Nanotechnology
-
language:en
-
Short-container-title:j biomed nanotechnol
Author:
Wang Yuli1,
Zheng Jie1,
Hu Hongqi1,
Xing Zhenyi1
Affiliation:
1. Department of Neurosurgery, Xinxiang Central Hospital, The Fourth Clinical College of Xinxiang Medical University, Xinxiang 453000, Henan, China
Abstract
Objective: To evaluate the effectiveness of PEGylated (PEG) OX26 loaded with Selenium (Se) on locomotor activity and brain function and exploring underlying mechanism in animal model of subarachnoid hemorrhage (SAH). Methods: Thoracis spinal cord injury in Wistar rats
was used to induce SAH and intra-epidural injection of OX26-PEG-Se nanoparticles (NPs) was then applied. Locomotor function test was used to evaluate the behavioral outcome in addition, ELISA kit was used to evaluate the serum level of NSE and S100B. Immunofluorescent imaging was used to detect
the expression of eNOS and NT-1. In addition, NeuN staing was used to assess the neural damage. Results: The locomotor function of animals with SAH was significantly increase afgter treating with OX26-PEG-Se NPs. In addition, the expression levels of NSE and S100B were significantly
decrease after treating animals with OX26-PEG-Se NPs in comparison to sham operated animals. We observed that OX26-PEG-Se NPs decrease the neural damage and the level of NT-1, while increase the eNOS in brain. Conclusion: Intra-epidural injection of OX26-PEG-Se NPs improved the locomotor
activity and also inhibit the risk of neural damage through ET-1/eNOS pathway after subarachnoid hemorrhage.
Publisher
American Scientific Publishers
Subject
Pharmaceutical Science,General Materials Science,Biomedical Engineering,Medicine (miscellaneous),Bioengineering