Long Noncoding RNA_Plasmacytoma Variant Translocation 1 (LncRNA_PVT1) Regulates the Biological Behaviors of Bone Marrow Mesenchymal Stem Cells (BMSC) to Promote Cervical Cancer Growth Through miR-266

Abstract

To discuss the biological mechanism of lncRNA PVT1 on regulating BMSC and prompting the HPV growth through miR-266. The correlation between presentation of lncRNA PVT1 and HPV was analyzed. The HPV cells were transfected with lncRNA PVT1. The transfection efficiency was tested. These cells were detected through CCK8 test. The action of lncRNA PVT1 on the HPV cells’ cycle and apoptotic rate was tested with FCM. The correlation between lncRNA PVT1 and miR-266 was analyzed through Pearson correlation analysis. The presentation of mRNA was tested by RT-PCR. The presentation level of miR-266 in BMSC and HPV cells was analyzed through PCR. The presentation of lncRNA PVT1 in HPV tissue and cell was increased notably. The apoptotic rate was increased and proliferative rate was reduced through the transfection with lncRNA PVT1. The miR-266 was one kind of gene with differential presentation. It was reduced notably in HPV cells. There was negative correlation between lncRNA PVT1 and miR-266. The proliferation of HPV cells was related closely with the increasing of the presentation of lncRNA PVT1. The gene presentation could be affected through the targeted interaction with miR-266. It was conducive to BMSC growing into HPV. It could provide target spot for the prevention and control for HPV.