Effects of Adrenomedullin (ADM)-Modified Bone Marrow Mesenchymal Stem Cells (BMSCs) Transplantation on Cardiac Function and Matrix Metalloproteinase Levels in Rats with Heart Failure

Abstract

After ADM gene treatment, bone marrow mesenchymal stem cells (BMSCs) were transplanted into rats with heart failure to study its effect on rat heart function and matrix metalloproteinases (MMPs) expression. The rats were assigned into control group, BMSCs group and ADM group. 4 weeks after transplantation, the rats’ cardiac function indexes were detected and the levels of MMP-2 and MMP-9 was measured by western blot. Echocardiography analysis showed significant differences between groups (except LVDd) (P < 0.05). BMSCs and ADM groups had significantly lower LVEF and LVFS levels than control group (P < 0.05) with a significantly higher level in ADM group than BMSCs group (P < 0.05). However, BMSCs and ADM groups presented a significantly higher LVDs (P < 0.05) without difference between them (P > 0.05). Interestingly, no difference of LVDd was found among groups (P > 0.05). MMP-2 and MMP-9 levels in BMSCs group were 0.389±0.021, 0.512±0.018, respectively, which were significantly elevated compared to control group (P < 0.05); MMP-2 and MMP-9 protein level in the ADM group was 0.440±0.018 and 0.539±0.032 respectively, which was significantly higher than control group (P < 0.05). Compared with BMSCs group, ADM group had significantly increased protein levels of MMP-2/9 (P < 0.05). In conclusion, ADM-modified BMSCs transplantation can significantly inhibit MMP-2 and MMP-9 level, thereby improving the heart function of rats with heart failure.