DHCR24 (24-Dehydrocholesterol Reductase) Associated in Modulating Steroid Biosynthesis Pathway Regulates the Differentiation of Chicken Embryonic Stem Cells into Male Germ Cells

Abstract

Spermatogonia stem cells (SSCs) have become one of the hotspots in modern life science research in the 21st century because of the broad application prospects in medicine, biology and animal breeding. Studies have shown that steroid biosynthesis signaling pathway is involved in the multiple cell differentiation process, but the formation of SSCs is not clear. DHCR24 proved in our outcome that it play an important part in steroid biosynthesis. Without the absent of DHCR24, CYP7A1 and PTCH2 are not keeping the expression of downstream genes. It’s the downregulation of the steroid biosynthesis pathway which lead to the decrement. What’s more, the steroid biosynthesis pathway could make it easy for the differentiation of embryonic stem cells (ESCs) is proved by qRT-PCR, immunofluorescence and flow cytometry analysis. All things considered. The above mentioned outcomes has lead to a model in which DHCR24 plays an important part in regulating ESCs differentiation by curing the activities of steroid hormones synthesis.