Regulation of Colon Cancer Cells Biology by Long Non-Coding RNA Metastasis Associated Lung Adenocarcinoma Transcript 1 (LncRNA MALAT1) via Targeting miR-184

Abstract

Colon cancer remains a serious threat to human, as lncRNAs and miRNAs are found as crucial components for colon cancer progression and metastasis. But the role of lncRNA MALAT1 in colon cancer remains elusive. Herein, we aimed at investigating its function in the colon cancer and exploring the interaction between MALAT1 and miR-184. We collected tumor tissue specimens from patients with colon cancer and determined the expression of MALAT1 and miR-184 using RTqPCR. Functional experiment was established to detect the impact of MALAT1 siRNA or miR-184 inhibitor, followed by transwell assay and scratch experiment to assess colon cancer cell migration and invasion. Bioinformatics software and luciferase reporter system analyzed the targeting relationship between MALAT1 and miR-184. MALAT1 was up-regulated in colon cancer tissue, while miR-184 was poorly expressed and they were correlated with the clinicopathology of colon cancer (P <0.05). miR-184 was indicated as the target miRNA of MALAT1. Moreover, silencing of MALAT1 up-regulatedthe expression of miR-184 when activating Caspase3 activity, inhibiting Bcl-2 expression, increasing Bax expression. And MALAT1 siRNA inhibited cancer cell migration and invasion (P <0.05). However, transfection of miR-184 inhibitor abrogated these effectsinhibitor, when increasing MALAT1 expression. Collectively, we demonstrated that miR-184 is the target miRNA of Lnc-RNA MALAT1 and MALAT1 promotes colon cancer cell progression through inhibition of miR-184.