Effects of Hederin (Hed) on Alveolar Bone Microstructure During Tooth Movement in Rats

Abstract

Purpose: To discuss effects of Hederin (Hed) to tooth movement process in rats. Materials and methods: 54 rats were divided into Model and Hed groups at 7 d, 14 d and 21 d, establishment of rat tooth movement model, the Hed and Model group injected Hed [5 mg/(kg· d)] and equal volume normal saline intraperitoneally respectively, Take the medicine regularly every night. After 14 days, 9 rats in each group were killed, BV/TV, Tb. SP and trabecular number (Tb. N) were measured by Mirco CT. Using TRAP staining and HE staining to observe osteoclasts number and pathology change. The relative protein expressions were measured by SP staining. Results: Compared with Model group, BV/TV and Tb.N were significantly down-regulation and Tb.Sp was significantly up-regulation in Hed group (P < 0.05, respectively); meanwhile, tooth movement and osteoclast number were significantly improved in Hed groups at 7 d, 14 d and 21 d (P <0.05, respectively). By SP staining, compared with Model group, ADRB2 and RANKL proteins expression were significantly enhanced at 7 d, 14 d and 21 d (P <0.05, respectively). Conclusion: Hed could promote alveolar bone resorption and increase the expression of ADRB2 and RANKL during orthodontic tooth movement.