Identification, Biological Characteristics and Semi Artificial Cultivation Experiment of Entoloma clypeatum

Abstract

Entoloma clypeatum (L.) P. Kumm., also known as Rhodophyllus clypeatus (L. fr.) Quel, is an ectomycorrhizal fungus that grows with Prunus mume. In this study, a strain of E. clypeatum was identified and characterized using morphological and molecular methods. Furthermore, the biological characteristics of the strain were observed, and a semi artificial cultivation experiment was conducted. The results showed that, the strain MBQM10168 was identified to be Entoloma clypeatum. The indices including the morphology, growth, and mycelial density of E. clypeatum exhibited significant differences under different conditions such as culture media, pH, growth temperature, carbon and nitrogen sources, and air permeability. However, it’s growth was not sensitive to light. The optimal growth conditions for E. clypeatum mycelium were cultured with branches of P. mume and cottonseed hulls culture media, with a pH range of 4–8, temperature range of 18–28 °C, along-side specific sources of carbon and nitrogen. These include cellobiose, sucrose, and sodium acetate as carbon sources, and lysine, diammonium citrate, methionine, and ammonium sulfate as nitrogen sources. In addition, with cotton plug for sealing method. Overall, this study demonstrated the feasibility of semi-artificial cultivation of E. clypeatum under suitable conditions, which is expected to promote the comprehensive utilization of the P. mume plant area and improve the economic values.