Application of solid-phase microextraction and LCMS/MS for the detection of pesticide residues

Abstract

Aiming at the organophosphorus pesticide residues in tea, a method integrating solid-phase microextraction and liquid chromatography-mass spectrometry was investigated for the detection of organophosphorus pesticide residues in tea. Firstly, the conditions in the process were optimized; then the method was established to analyze the organophosphorus pesticides in tea; finally, the method feasibility was verified by using actual sample determination. In terms of the results, the standard curve correlation coefficients of the solid-phase microextraction and liquid chromatography-mass spectrometry methods used in the study were greater than 0.99, while the average recoveries ranged from 72 to 109% with the relative standard deviations (RSDs) of less than 9.0%. This indicates that the method has a good linear range, low detection limit and high recovery. In the application validation in real samples, the concentrations of organophosphorus pesticide residues in tea were within the safe range. The method was validated to be suitable for the monitoring and control of organophosphorus pesticide residues in tea with low cost and simplified sample preparation.