Oligomerization of IHF protein in the presence of metal cations-Reference-Cited by-同舟云学术

Oligomerization of IHF protein in the presence of metal cations

Published:2024-07-19 Issue:2 Volume:69 Page:268-276
ISSN:0023-4761
Container-title:Кристаллография
language:
Short-container-title:Kristallografiâ

Author:

Gordienko A. M.¹,Dadinova L. A.¹,Petoukhov M. V.¹²³,Mozhaev A. A.¹²,Manuvera V. A.⁴⁵,Lazarev V. N.⁴⁵,Shtykova E. V.¹

Affiliation:

1. Shubnikov Institute of Crystallography of Kurchatov Complex of Crystallography and Photonics of NRC “Kurchatov Institute”

2. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

3. A.N. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences

4. Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency

5. Moscow Institute of Physics and Technology

Abstract

The oligomeric state of the nucleoid-associated protein IHF (integration host factor) plays a significant role in organizing and compacting bacterial nucleoids, as well as in the process of bacterial resistance to adverse environmental conditions, including antibiotics. Although IHF was identified more than 25 years ago, the molecular mechanisms of its involvement in such processes remain poorly understood. In this study, using small-angle X-ray scattering, various oligomeric forms of IHF were first identified in aqueous solution depending on the presence of metal cations. It was found that the presence of Mg2+ and K+ ions inhibits the formation of high-order oligomers. The obtained data can be useful in developing strategies to overcome bacterial resistance to drugs.

Publisher

The Russian Academy of Sciences

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